Protagonists
- Lady LovelyLocks - The series' main protagonist, and Lady is her given name. She wears a pink dress and has blonde hair with streaks symbolizing her royalty in three colors: soft pink (dawn), gold (sun) and lavender (twilight). She is the princess of the Kingdom of LovelyLocks as well as its main source of power and life. She is a very loyal, brave, kind, selfless, polite, resourceful, optimistic, dependable and friendly individual who always treasures her people and her home. (Paired with three bunny-like Pixietails for the first toy line.)
- Maiden FairHair - A maiden and best friend of Lady LovelyLocks who wears a blue dress and whose light brown hair is worn in waves. A natural beauty, party-planner, daydreamer and painter, she is very serene, cool-headed, and intelligent, but would sometimes lose track of something important and/or, to the extent, her sense of direction. (Paired with three squirrel/chipmunk-like Pixietails for the first toy line.)
- Maiden CurlyCrown - Another maiden and friend of Lady LovelyLocks who wears a yellow dress and curly red hair. A funny and imaginative girl with a big heart, she loves making up stories, styling her hair, and collecting bugs, but is somewhat impulsive and, despite being quite smart, would sometimes get herself into trouble. (Paired with three bird-like Pixietails for the first toy line.)
- SilkyMane - Lady's kind-hearted, trusty royal-purple horse who always understands her thoughts and feelings. (Paired with five bunny-like Pixietails for the toy line.)
- SilkyPup - Lady's happy-go-lucky, yet trouble-prone pink pet puppy who has a secret crush on the Prince. (Paired with three bird-like Pixietails for the toy line.)
- ShiningGlory - a blind, yet very wise and powerful sorcerer and wizard, and the beloved mentor to Lady and her friends. He often aids Lady with his magic. It appears that he adopted the Prince after he was cursed. When inside a dimensionally transcendental crystal in his castle, ShiningGlory has his eyesight, but when outside, he is blind. The character was never produced for the toy line.
- Prince StrongHeart - originally a human, but was turned into a dog known as "Prince" as a result of a dreadful curse. He often visits the castle gardens since being adopted by ShiningGlory. At times, the opportunity for him to turn human again arises; however, he always foregoes that chance, opting to help his companions instead, such as coming to Lady's rescue when she is in danger. He is not often seen in his human form, though in ShiningGlory's crystal, StrongHeart appears human. For the toy line, he was produced as a brown-haired human, whereas he appears blond in the show. (Paired with two to three mouse-like Pixietails for the toy line, depending on the variant.)
Antagonists
- Duchess RavenWaves - The series' main antagonist. She wears a purple dress and, like Maiden FairHair, her black hair is styled in waves. She is the mean, jealous, cruel, stubborn, selfish, domineering, cold, and conniving ruler of Tangleland, as well as the main archenemy of Lady LovelyLocks. Duchess always plots dastardly schemes to take over the Kingdom of LovelyLocks for herself, and believes that cutting off a piece of her rival's hair would help her reach that goal. Despite all of her negative traits she is not evil, and on a few occasions actually helps Lady when it is to their mutual advantage.
- HairBall - a former apprentice of ShiningGlory who is now in the employ of RavenWaves. Deemed untrustworthy by good people, he specializes in magic and is quite practical when it comes to making and causing trouble. He also acts miserable when he feels defeated; however, this makes him quite sympathetic to his peers. The animated show depicts HairBall as a non-human troll-like creature, whereas most print media depicts him as a human dwarf. The character was never produced for the toy line.
- Comb Gnomes - creatures with big ears and wild-looking hair and fur who are henchmen of Duchess RavenWaves and accomplices to HairBall. They are mischievous and mean-spirited, yet lazy and irresponsible when executing their plans, and become upset when their part of a planned scheme fails. They are also archenemies to the Pixietails, as they all have magical powers, of which theirs is a lightning attack. Their names are Snags, Dulla and Tanglet. Unlike the many different Pixietails, these three are the only Comb Gnomes ever to be seen in the animated show; print media depicts many more of the species living in Tangleland. Only two different Comb Gnomes were supposed to have been produced for the toy line (hot pink and dark purple tails), though due to quality control issues, a few color variants exist.
The Pixietails
The Pixietails are colorful, fairy-like animals, who help Lady LovelyLocks whenever she is in need of a solution to a problem or whenever someone is in trouble. Lady's (and SilkyMane's) Pixietails are bunny-like, FairHair's Pixietails are squirrel/chipmunk-like, and CurlyCrown's (and SilkyPup's) Pixietails are bird-like. In the toy line, Prince StrongHeart's (and the baby dragons') Pixietails are mouse-like. In the animated show, Lady's bunny-like Pixietails are able to fly, whereas in print media only bird-like (and later butterfly-like) Pixietails are depicted flying.
The toy line's expansion into the Enchanted Island series later introduced fish-like, turtle-like, snail-like, and seahorse-like Sea Magic Pixietails, each with tightly-curled strands of power-curls added to their regular tails. Lady was paired with three fish-like, FairHair with two turtle-like, and CurlyCrown with two snail-like Pixietails. A few of the bunny-like and bird-like Pixietails were later given power-curls as well, sold with ballerina variants of Lady LovelyLocks and Maiden FairHair, and in separately available fashion-packs that also featured more of the Enchanted Island's Sea Magic Pixietails.
The toy line's final expansion added six butterfly-like Pixietails with sparkly strands to its lineup of flower-scented CurlyKittens, and three to a "Sparkle Pretty LadyLovelyLocks" doll.
Named Pixietails include:
- Pixieshine - A blue bunny-like Pixietail under the ownership of Lady LovelyLocks.
- Pixiebeauty - A purple bunny-like Pixietail under the ownership of Lady LovelyLocks.
- Pixiesparkle - A pink bunny-like Pixietail under the ownership of Lady LovelyLocks.
Dragons
Dragons exist in the Kingdom of LovelyLocks. They can breathe fire and have wings to fly with. Instead of hatching from eggs, newborn dragons are born from the Dragon Tree. A plant called Dragon's Hair has some connection. For the toy line, the baby dragons' Pixietails are mouse-like.
- LongCurl - The large, yellow mother dragon of the dragon triplets (not produced for the toy line).
- SweetCurl - Pink baby dragon (paired with a yellow-and-orange-pink mouse-like Pixietail for the toy line).
- MerryCurl - Purple baby dragon (paired with a blue-and-yellow-pink mouse-like Pixietail for the toy line).
- BouncyCurl - Green baby dragon (paired with a pink-and-blue-green mouse-like Pixietail for the toy line).
Hide'N'Seeks
Only appearing in the toy line and some print media, these three shy, humanoid fairies are described to hide behind their long hair, among the colorful flowers and shrubs in the valley between the Kingdom of LovelyLocks and Enchanted Island. They're "so bashful that they only peek out when their friends are near." The toy line first introduced them with bird-like Pixietails, but quickly changed the pairing to Enchanted Island's (turtle-like, snail-like, and fish-like) Sea Magic Pixietails.
- PerkyPeek - Pink-haired fairy with purple streaks, and a yellow turtle-like Sea Magic Pixietail.
- SunnyPeek - Blue-haired fairy with green streaks, and a pink snail-like Sea Magic Pixietail.
- PearlyPeek - Purple-haired fairy with pink streaks, and a blue fish-like Sea Magic Pixietail.
LilyTops
Only appearing in the toy line's Enchanted Island expansion, these three seal-like "water babies" are described to be "hiding under giant lilypad hats, and love to play in the water." Produced to float, they can "ferry the Sea Magic Pixietails around the waters of Enchanted Island." The toy line paired them with Enchanted Island's (turtle-like, snail-like, and fish-like) Sea Magic Pixietails.
- LilySplash - Blue seal-like creature with a yellow lilypad hat, and an orange-and-pink fish-like Sea Magic Pixietail.
- LilySprinkle - Yellow seal-like creature with a purple lilypad hat, and a blue-and-pink turtle-like Sea Magic Pixietail.
- LilyBubble - Purple seal-like creature with a pink lilypad hat, and a yellow-and-orange seahorse-like Sea Magic Pixietail.
Mermaids
- Maiden GoldenWaves - only appearing in the toy line's Enchanted Island expansion and some print media, this maiden is a light-skinned, blonde-and-blue-haired mermaid in a blue tail-like dress, and two seahorse-like (purple-pink and orange-yellow) Sea Magic Pixietails.
- Maiden MistyCurls - only appearing in the toy line's Enchanted Island expansion, this maiden is a dark-skinned, black-and-purple-haired mermaid in a purple tail-like dress, and two seahorse-like (pink-orange and blue-purple) Sea Magic Pixietails.
CurlyKittens
Only appearing in the toy line's final expansion, six colorful kittens appear in the Kingdom of LovelyLocks to "make the yearly Flower Ball even more fragrant with their different flower scents". Each is paired with one sparkly-tailed, butterfly-like Pixietail.
- PinkyPaws - Pink, rose-scented kitten with blue-and-purple butterfly-like Pixietail.
- PurplePurr - Purple, lilac-scented kitten with blue-and-pink butterfly-like Pixietail.
- PeachyPuff - Orange, lilly-scented kitten with green-and-yellow butterfly-like Pixietail.
- SunnySoft - Yellow, buttercup-scented kitten with pink-and-purple butterfly-like Pixietail.
- SapphireShy - Blue, carnation-scented kitten with yellow-and-pink butterfly-like Pixietail.
- CreamyCoat - White, gardenia-scented kitten with purple-and-pink butterfly-like Pixietail.
